Screening of anticancer potential of leaf aqueous extract fromPulicaria incisa growing wild in Palestine

Authors

  • Lubna Abdallah Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine, P400
  • Ghadeer Omar Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine, P400
  • Shaima Abu Khdeer Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine, P400
  • Hadeel Heeh Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine, P400
  • Deena Khateeb Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine, P400
  • Noor Salameh Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine, P400

DOI:

https://doi.org/10.56042/ijnpr.v14i3.5404

Keywords:

Anticancer, Cytostatic, Cytotoxic, Extract, Pulicaria incisa

Abstract

The anticancer potential of Pulicaria incisa aqueous extract was estimated by measuring its cytotoxic and cytostatic effect on human cervical cancer (Hela), human colorectal adenocarcinoma (HT-29), and skin melanoma cancer (B16–F1) cell lines. This effect was investigated by the exposure of cancer cell lines to the aqueous extract of P. incisa at different concentrations (5, 2.5, 1.25, 0.625, 0.3215 mg/mL) for 24 and 72 hours. The results showed that the studied extract inhibited the growth of the various cell lines, eventually leading to cell death, as shown by both MTT and LDH assays. The extract of P. incisa displayed the strongest cytotoxic effect against HT-29 cells at high concentrations at which viability was nearly zero %, while the strongest cytostatic effect of the prepared extract was on the B16–F1 cells at high concentrations. Nonetheless, P. incisa extract gave exciting results on Hela cells with a cytotoxic effect at high concentrations and a cytostatic effect at low concentrations. In conclusion, the cytotoxic and cytostatic effect of P. incisa aqueous extract against the three cell lines is due to the presence of several phytochemicals. So, using this plant species as a source for antitumor agents alone or in combination with other agents for cancer treatment is proposed.

Downloads

Published

2023-10-25