Computational analysis of Linum usitatissimum-derived peptides: Toward therapeutic applications

Abstract

The food proteome contains diverse bioactive peptides, each offering distinct health advantages for the cardiovascular, nervous, immune, and gastrointestinal systems. This study researches the rich repository of bioactive peptides in flax seeds (Linum usitatissimum), focusing on their potential health benefits across diverse physiological systems. Leveraging in silico techniques, a comprehensive analysis of 11 protein sequences sourced from UniProt was conducted, utilising enzymatic digestion to generate 160 non-repetitive, non-toxic peptides employing pepsin, trypsin, chymotrypsin, and combined enzymatic strategies via BIOPEP database. The evaluated peptides were scrutinised for a spectrum of biological properties encompassing antimicrobial, antitubercular, and anticancer attributes, alongside an investigation into their immunogenic potential. Docking studies further elucidated the interactions between these peptides and their respective targets, shedding light on their mechanisms of action. The findings unveiled a subset of peptides exhibiting substantial potential across multiple properties, establishing flax seeds as a compelling source of bioactive peptides. This in silico predictive approach serves as a vital precursor, offering cues for designing targeted experimental methodologies aimed at extracting therapeutic peptides. The implications of this research extend to the development of functional foods or pharmaceuticals harnessing the bioactive potential of flax seed-derived peptides for promoting human health and well-being.