Assessment of in vivo wound healing potentiality of Trapa natans L. leaves on different wound models in rat

Abstract

The primary goal of this study was to assess the wound healing potentiality of Trapa natans L. Excision and incision models were used to evaluate wound healing activity. For the excision model experiment was conducted for 21 days and for the incision model experiment continued for 10 days. Experimental animals were divided into four groups, the control group received simple ointment, the animal of the standard group treated with 0.2% w/w soframycin ointment, and the test groups received T. natans extract ointment (2.5% w/w & 5% w/w). Wound healing was determined by the rate of contraction, the time required for epithelization, collagen cell formation, the amount of hydroxyproline present in the wound site, and wound-breaking strength. Experimental results of the test showed a comparatively higher rate of contraction, lesser epithelization period, and higher hydroxyproline amount present in the wound area. These results were strengthened by the histopathological studies of the healing area tissues. When the standard and test groups were compared to the control group, statistically significant data were found (p<0.001). The test group had better wound-healing properties as compared to the control group. From the result of this study, it was revealed that T. natans L. is an excellent herbal alternative for treating wounds.