From synthesis to bioactivity: A comprehensive study of Cu-based biocidal tool


  • Dr N Anantha Krishna Department of Mechanical Engineering, Mohan Babu University erstwhile Sree Vidyanikethan Engineering College, Tirupati 517 102, India
  • Dr Y V Mohana Reddy Department of Mechanical Engineering, GPREC, Kurnool 518 001, India
  • Dr B Chandra Mohan Reddy Department of Mechanical Engineering, JNTUA, Ananthapuramu 515 002, India



Biocidal, Copper, Fermentation, Metallurgy, Organic, Rasa shastra


The present study reports on the biogenic synthesis of a copper-based biocidal material through the fermentation of gruel, a traditional non-alcoholic beverage. This process may involve a bio-beneficiation mechanism, in which the indigenous microorganisms in the ferment interact with the material. X-ray diffraction analysis confirmed the powder's crystalline copper composition. Fourier-transform infrared spectroscopy revealed the crucial role of organic acids in the capping process. Transmission electron microscopy, ultraviolet-visible spectroscopy, and antimicrobial susceptibility tests were conducted to characterize the powder. Furthermore, the biocidal material was combined with the anticancer drug curcumin to explore its additional anti-proliferative effects, including apoptosis, on human hepatocellular carcinoma cell lines in vitro. These findings highlight the potential of this biogenic copper material as a promising candidate for biomedical applications.