Isolation and identification of few fatty acid esters from the aerial roots of Rhaphidophora aurea twined over different host trees

Abstract

Plants are the store houses of various secondary metabolites and bio-essential natural products. Several therapeutic agents are isolated from Rhaphidophora aurea. The aerial roots of the mentioned plant twined over different trees were explored. Sequential extraction (Polar to non-polar solvents) was carried out for the aerial roots of Rhaphidophora aurea twined over different host trees. Each extract was subjected to solvent-solvent fractionation. Through Thin-layer chromatography and column chromatography, the fatty acid compounds were identified and isolated. The isolated compounds were characterized through recording UV, IR, GC-MS/MS (Thermo), and 1D and 2D NMR techniques. Six fatty acids viz. 4-oxo-tricosanoic acid icosyl ester, butyl octadecanoate, dodecanoic acid dodec-3-enyl ester, octacosa-23, 26-dien-12-one, ethyl cis-6-octadecenoate and 15,18- dotriacontadienoic acid and methyl ester was isolated and characterized by analytical characterization techniques.