Evaluation of Argyreia speciosa (Linn.) sweet activity on human dental pulp stem cells

Neurodifferentiation activity of Argyreia speciosa (Linn.) sweet

Abstract

Indian traditional medicine specifically defines a class of substances including Argyreia speciosa (Linn.) sweet (AS) that modulates the central nervous system. Nevertheless, the precise mechanism of action of AS remains unknown. The objective of this investigation was to determine the neural lineage and gene expression by utilizing Nestin and beta-tubulin. A standardized aqueous extract was produced by processing an authentic sample of AS. Stem cells were identified and characterized. The drug's viability was assessed using the MTT assay. We utilized AS to stimulate neural differentiation in human dental pulp stem cells (hDPSCs). We employed RTPCR to evaluate gene expression in hDPSCs using markers such as Nestin and beta-tubulin. The MTT assay demonstrated that the 10 µg/mL concentration facilitates cell differentiation into trilineage differentiation. Neural differentiation was observed in the treated group, which received AS and neurogenic media. The neurogenic potential was assessed by RT-PCR, which revealed significantly greater Nestin expression in the experimental group comparable to the control group. The aqueous extract of AS met the previously stated pharmacokinetic requirements. Neural differentiation was observed within seven days. The sole indicator of gene expression was the Nestin neural marker. A. speciosa may be employed as a complementary medication to address a diverse array of neurological disorders.