Synthesis, characterization and antibiotic capabilities of microspheres loaded with essential oils

Abstract

The purpose of the current work was to synthesize, describe, and assess the antimicrobial properties of microspheres against diverse microorganisms such as Bacillus subtilis, Pseudomonas aeruginosa, and Aspergillus niger. The production of polymeric microspheres using essential oils, as well as testing the impact of such microspheres on several biological properties was carried out. The ionic gelation approach was used in the laboratory to create CEO-loaded polymeric microspheres, which were then improved for use in subsequent experiments and characterized by Particle Size Analyzer, Scanning Electron Microscopy, and Transmission Electron Microscopy. The DPPH Assay and antibacterial activities were used to enhance the study of polymeric microspheres loaded with essential oils. Additionally, to observe the per cent encapsulation efficacy, synthetic microspheres have been used. Microspheres obtained during the experimental process were in size ranging from 80 to 290 nm, depending on the amount of drugs and polymers contained. Microspheres could be spherical in shape and aggregations at specific sites, according to SEM and transmission electron microscopy. The range of encapsulation efficiency for various concentrations was 6 to 25%. By using the DPPH assay, it was observed that the antioxidant activity lies between 28 to 64%. When anti-microbial activities were performed it was observed that the incorporation of essential oils into polymers showed tremendous potential against microbes. In the current research, the essential oils-loaded polymeric microspheres showed a significant impact on a variety of microorganisms. Additionally, these microspheres exhibited noticeable radical scavenging activities. In summary, it may be claimed that certain physiochemical alterations can be used to leverage the diverse activities of these microspheres for experimental uses in the future.