Antiproliferative potential of Sargassum binderi Sonder ex J. Agardh mediated biogenic silver/silver chloride nanoparticles on EAC, MCF-7 and HCT116 cells

Abstract

Biosynthesis of Ag/AgCl-NPs from Sargassum binderi extract was confirmed by colour changes and a sharp peak at
416 nm detected by UV-visible spectrophotometer. It was characterized by Transmission Electron Microscopy (TEM), Energy Dispersive X-ray (EDX) spectrophotometer, X-ray powder Diffraction (XRD) and Fourier-Transform Infrared Spectroscopy (FTIR). Mild toxicity of the synthesized Ag/AgCl-NPs was determined by the brine shrimp nauplii cytotoxicity assay. IC₅₀ values of 19.73, 25.56, and 14.00 µg/ml showed the growth inhibition of Ehrlich Ascites Carcinoma (EAC), human breast cancer (MCF-7) and human colon cancer (HCT116) cells by Ag/AgCl-NPs, respectively. By analyzing cell morphological study using annexin V and caspase-3 inhibitor, initiation of apoptosis in EAC cells was confirmed. The results were further supported through the expression of several genes, where, p53 and Bax genes became activated and Bcl-X and NFκB genes were suppressed.