GCMS analysis and mosquitocidal effects of petroleum ether extract of Datura stramonium and Morus alba against Aedes aegypti

Abstract

Aedes aegypti (A. aegypti) causes millions of deaths every year being the vector of many human diseases like dengue virus, yellow fever, chikungunya, and Zika. Phytochemicals area blend of secondary metabolites which are eco-friendly as well as controlling agents against A. aegypti and therefore diseases spread by them. Chromatographs of the GC-MS analysis of the petroleum ether extract of Datura stramonium and Morus alba leaves showed the presence of various compounds. Phytol, tetratetraacontane, pentanoic acid in D. stramonium and mibemycin b,13-chloro-5-o-demethyl-28-deoxy-6,28-epoxy-25-1{1-mwthyl propyl}-(6r-13s,25A(S), acetic acid, 17-{4-chloro-5-methylhexyl}-4,4,10,13,14-pentamethyl-2,3,4,5,6,7,10, 11,12,13,14,15,16,17-tetradecahydro-1-phenanthyl, lup-20{29}-en-3-one in M. alba were the main constituents. The study screened for larvicidal, ovicidal, ovipositional deterrent, and adulticidal activity. The leaves of both plants were collected, washed in tap water, air dried in shade, powdered, and subjected to Soxhlet extractions with petroleum ether solvent. Seven different concentrations of D. Stramonium and M. alba extracts were performed to get LC₅₀.D. stramonium and M. alba, recorded LC₅₀ at 80.42 and 118.627 mg/mL respectively after 24 h. Whereas LC₅₀ after 48 h is recorded at 73.04 mg/mL in D. stramonium and 111.68 mg/mL in M. alba. The results indicated that D. stramonium is a better mosquitocidal agent than M. alba.