Synthesis, characterization, antimicrobial and antioxidant screening of novel Oxazepines

Abstract

Oxazepines (4a–4e) were synthesized by condensing aromatic aldehydes with sulfamethoxazole in the presence of chloroform and methanol, resulting in the formation of Schiff bases (3a–3e). The Schiff bases were subsequently condensed with maleic anhydride in dry benzene. The synthesis was validated through FTIR, ¹H NMR, mass spectrometry and elemental analysis. The compounds of interest were assessed for their antimicrobial properties against Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Staphylococcus aureus through the Kirby-Bauer disc diffusion technique. Furthermore, the fluorescence method was employed to screen for antioxidant activity. The pharmacokinetics of the compounds were evaluated using computational studies conducted through SwissADME. Additionally, molecular docking studies were performed on thymidylate synthase A, revealing that the compounds exhibited favorable interactions with the target.