Exploration, Molecular Characterization, And Optimization Of Novel Lipase-Producing Microalgae Chlorella Sorokiniana LGS2 Isolated From Lake Water

Abstract

This study investigates the production and optimization of lipase enzymes from microalgae isolated from lake water. Various microalgal strains were isolated, cultured under controlled conditions, and evaluated for lipase activity using qualitative assays such as tributyrin agar, Rhodamine B, and bromothymol blue plate methods. Molecular identification via 16S rRNA chloroplast sequencing revealed Chlorella sorokiniana LGS2 as the most efficient strain, with its sequence deposited in GenBank under accession number PQ583068. Lipase production was induced in BG-11 medium supplemented with olive oil, and enzymatic activity was assessed through the hydrolysis of p-nitrophenyl acetate. Optimization of physicochemical parameters, including light intensity, temperature, pH, agitation, and substrate concentration, was conducted to maximize lipase production. This research highlights C. sorokiniana LGS2 as a novel and promising source for lipase production, providing a valuable foundation for future biotechnological and industrial applications.