Scanning electron microscopy study of Raillietina echinobothrida Megnin, 1880 (Cestoda) and Syphacia obvelata Rudolphi, 1802 (Nematoda) exposed to Cyperus compressus (L.) (Cyperaceae) and its GC-MS study

Anthelmintic efficacy study of Cyperus compressus (L.)

Abstract

The roots of Cyperus compressus (Cyperaceae) have traditionally been used to treat intestinal helminthiasis in India. This study was designed to examine the abnormalities caused on Raillietina echinobothrida Megnin, 1880, and Syphacia obvelata, Rudolphi, 1802, on exposure to the root methanolic extract of C. compressus, using scanning electron microscopy. Worms were placed in extract concentration of 30mg/mL and on paralysis, worms were dehydrated in acetone grades and images were taken. The extract was then processed for GC-MS study to detect the compounds present in it. The images portrayed damaged microtriches in R. echinobothrida and loss of annulations and wrinkled cuticle were observed in S. obvelata. Compared to the damages caused by the reference drug, the results were milder. GC-MS study revealed 30 prominent peaks where 7-Methyl-Z-tetradecen-1-ol acetate, was the most abundant compound which was detected at retention time 20.48 and with a relative abundance of 60.49. Its efficacy could be contributed to these detected compounds in the extract.