Protective effects of safranal against subchronic thinner inhalation induced oxidative stress in rats

Abstract

This study was carried out to determine the effects of subchronic thinner inhalation on the oxidant− antioxidant balance,
the relation between toxicity and oxidative stress, and a possible protective effect of α-lipoic acid against thinner toxication
in rats. Sprague−Dawley rats were divided into 4 groups as follows: control (Gr. I), safranal (Gr. II), thinner (Gr. III), and
thinner+safranal Gr. IV). Each group consisted of 10 rats, and the study lasted for 8 weeks. After completing the animal
studies malondialdehyde (MDA), reduced glutathione (GSH), toluol, 8OHdG (8 hydroxy 2 deoxyguanosine), protein
oxidation, nitric oxide metabolites (NOx), total antioxidant capacity (TAC), total oxidant capacity (TOC), glucose, total
cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, WBC, VLDL, RBC, HCT, Hb, aspartate amino transferase
(AST), and alanine amino transferase (ALT) levels were determined from blood specimens of the rats. Brain and lung
tissues were also examined histopathologically. The data obtained from the study were statistically analyzed using SPSS,
and both ANCOVA and Bonferroni tests were performed. P <0.05 was accepted as statistically significant. The results
indicated that safranal had a protective and balancing effect against thinner inhalation oriented complications in rats.