Genotoxic effect of manganese and nickel doped zinc ferrite (Mn0.3Ni0.3Zn0.4Fe2O4) nanoparticle in Swiss albino mouse Mus musculus

Abstract

Manganese and Nickel doped Zinc Ferrite (MNZF) nanoparticle Mn_0.3Ni_0.3Zn_0.4Fe₂O₄ is used in fabrication of room temperature (25-30ºC) NH₃ gas sensor in large scale industries. However, there are no studies available on its toxic effects. Hence, in the present study, we assessed the genotoxic effect of various doses (125, 250 and 500 mg/kg) of the MNZF nanoparticle (NP) in Swiss albino mice Mus musculus employing the chromosomal aberration test, micronucleus test and single cell gel electrophoresis assay (comet assay). The NP was orally gavaged for 15 consecutive days. Dose-dependent study was conducted at 24 h after the last dose of gavage and time-dependent response was studied for 250 mg/kg at 24, 48 and 72 h of treatment. All the parameters employed showed a statistically significant dose-dependent increase of genetic damage indicating the genotoxic effect of this NP in Swiss albino mice. Proper precautions should be undertaken on handling this NP to avoid contact with it either through respiration or ingestion.