In vitro anticancer and apoptotic activity of edible mushroom Lepista nuda (Bull.) Cooke on leukemia and breast cancer compared with protocatechuic acid, paclitaxel and doxorubicin

Abstract

Macro fungi are used as food and for therapy for centuries. One such fungus Lepista nuda (Bull.) Cooke is reported to exhibit many biological activities while other species viz. Lepista inversa and L. sordida possess active substances. In this study, we prepared extractions depended on polarity increase from the edible mushroom Lepista nuda and studied its action on HL60 (leukemia) and MCF7 (breast cancer) cancer cell lines., We used PCA (protocatechuic acid), paclitaxel and doxorubicin as positive controls. Methanol extract was found effective on both cell lines. The extract showed fairly significant (IC₅₀ ~15 mg/mL) of biologic activity compared with drugs protocatechuic acid, paclitaxel and doxorubicin against HL-60 cell line with regard to both proliferation and apoptotic effects (>%75).