Effects of modified Bushen Huoxue Prescription combined with active immunotherapy on immunomodulatory functions and pregnancy outcomes of patients with unexplained recurrent spontaneous abortion

Treatment of unexplained recurrent spontaneous abortion

Abstract

To assess the effects of modified Bushen Huoxue Prescription combined with active immunotherapy on immunomodulatory functions and pregnancy outcomes of URSA patients. Ninety-six eligible URSA patients were randomly divided into control and experimental groups (n=48). Active immunotherapy was performed for control group, while experimental group were treated with modified Bushen Huoxue Prescription combined with active immunotherapy. Counts of peripheral blood helper T lymphocytes 17 (Th17) and regulatory T lymphocytes (Treg), levels of inflammatory factors interleukin-4 (IL-4), IL-10 and IL-17, and mRNA expression levels of retinoic acid-related orphan receptor γt (RORγt) and forkhead box P3 (FoxP3) were compared. Compared with before treatment, ratios of Th17/CD4⁺ cells and Th17/Treg, IL-17 level and RORγt mRNA level decreased, whereas ratio of Treg/CD4⁺ cells, IL-4 and IL-10 levels and FoxP3 mRNA level increased in both groups after treatment (P<0.05). After treatment, ratios of Th17/CD4⁺ cells and Th17/Treg, IL-17 level and RORγt mRNA level were lower in experimental group than those in control group, while ratio of Treg/CD4⁺ cells, IL-4, IL-10 levels and FoxP3 mRNA level were higher in experimental group (P<0.05). Modified Bushen Huoxue Prescription combined with active immunotherapy worked well for URSA to improve pregnancy outcomes and lower abortion rate.