The Evaluations of blood lipids, lipid peroxidation and antioxidant enzymes activities in women with uterine tumors

Abstract

The research aim was to study alterations in lipid spectrum, lipid peroxidation intensity and fatty acid (FA) spectrum in the blood of women with benign and malignant uterine tumors during the menopausal period. In case of malignant tumor, following changes were observed regarding saturated FAs: decreased myristic (3-fold) and palmitic acid (~1.3 fold) compared to control, but stearic acid was increased (1.13-fold). The decreased ratio between palmitic/stearic acids (C_16:0/C_18:0) by ~1.5-times was noted. In case of unsaturated FAs in malignant tumor samples, there was a decrease in oleic (2.9-fold), linolenic (1.5-fold), eicosatrienoic (3-fold), and arachidonic (1.3-fold) acid compared to control. The increased ratio between stearic/oleic acids (C_18:0/C_18:1) by 3.3-times was noted in malignant cases. Increase in lipid peroxidation intensification was associated with altered lipid spectrum composition (hyperlipidemia, disruption in the exchange rate of phospholipids). There was a negative correlation between lipid peroxidation intensity and phospholipids (r=−0.96±0.04). Total cholesterol level was higher in malignant cases. Total amount and activities of superoxide dismutase (SOD) and catalase (CAT) were decreased in malignant cases. The negative correlation between total cholesterol and SOD, CAT activities was r=−0.99±0.014. Amine-containing phospholipids were decreased, but choline-containing phospholipids levels were increased along with the severity of the disease. It is concluded that intensification of lipid peroxidation and alterations in lipid spectrum composition contributes to hyperlipidemia, disruption of phospholipids’ exchange rate and hypercholesterolemia. Thus, specific changes in these biochemical parameters can be useful in the differentiation of benign and malignant uterine tumors for menopausal women.