Rapid genomic DNA extraction for Soybean (Glycine max L. Merr) using modified CTAB protocol to obtain high-quality DNA

Abstract

Molecular and genetic analysis is one of the critical primary stages, starting with DNA extraction. DNA extraction is an essential preliminary step in molecular research. Extracting plant genomic DNA with CTAB buffer takes approximately three days to obtain high-quality DNA. There have yet to be reports regarding effective and efficient soybean genomic DNA extraction methods. This study aimed to obtain a rapid extraction method for soybean with high-quality DNA. This study used a modification of the CTAB method by shortening the duration its stages. The treatments tested were washing soybean leaf and callus organs with ethanol one and two times. The results showed that one-time washing using ethanol absolute obtained the highest average DNA concentrations in leaf and callus organs 458.72 ± 155 ng/μL and 363.87 ± 205 ng/μL, respectively. In addition, DNA purity was obtained 1.99 ± 0.16 and 1.86 ± 0.07, respectively, on A260/280 ratio. Good DNA quality was indicated by the absence of smears on the 1.5% electrophoresis gel. This study demonstrated that extracting plant DNA completed in 70 min to obtain the highest quality DNA.