Assessment of quality protein parameters of Zea mays L. through high throughput biochemical analysis

Abstract

Maize is one of the world’s mostly cultivated crops which are consumed by the humans. The quality of maize protein is a vital factor in combating human malnutrition, a problem that is rapidly increasing among children. This study focuses on enhancing maize's protein quality by increasing lysine and tryptophan concentrations while reducing zein content. Seventeen in-house maize lines were analyzed for key biochemical characteristics. Among these, the CML161 line stood out, with a total protein content of 11.831%. The tryptophan and lysine contents in CML161 were 0.68% and 3.3%, respectively. Additionally, the total starch content in CML161 was found to be 37.8%. The endosperm modification test showed 100% opaqueness in the CML161 variety. Based on these findings, it can be concluded that CML161 contains a quality protein content of 5.74%, making it a promising candidate for further studies aimed at addressing malnutrition.