Revealing genetic variation in mini core germplasm of urdbean (Vigna mungo (L.) Hepper)

Abstract

A set of 47 urdbean genotypes including 12 promising varieties and 34 popular land races and a popular OUAT variety “Ujala” (standard check) were characterized for genetic variation based on molecular markers and morpho-economic traits. The molecular marker- based genotyping revealed a tremendous higher level of polymorphism (97.05%) with high average PIC (polymorphic information content) value (0.75). ISSR 1357 was considered highly informative that revealed the highest PIC (0.87) and marker index value (MI:5.25). An 840 bp allele (band) was characteristic to Kantapada local - A, Kendrapada local-D, and Nayagarh local - C. Such genotype- specific finger-printing may serve for reliable varietal characterization and elimination of duplicates. The test genotypes were grouped into six distinct clusters. TU 10-13, LBG 623, TAU 1, OBG 33, LBG 17 and PU 31 were highly divergent. PU 31 had inherent high yield potential (˃5.0q/ha) with known YMV resistance. The above divergent high yielding test genotypes may serve as candidate varieties for further genetic improvement using recombination breeding.