Identification of a new MYBL2 isoform with distinct subcellular distribution

Abstract

Different splicing variants of genes may have different functions, and the identification of a new Splicing variant may broaden the role of the gene in many respects.  MYBL2 plays a crucial role in the regulation of cell growth and differentiation, cell survival and cell cycle progression. In human embryonic stem cells (hESCs), there are usually two splicing forms of MYBL2 reported, MYBL2a and MYBL2b. Here, we successfully cloned the novel splicing variant of the MYBL2, which is named MYBL2c in vitro. MYBL2c lacks exon 3 and exon 4, as well as most of the regions encoding the DNA-binding domain. More importantly, MYBL2cis located in both the nucleus and cytoplasm, while MYBL2a is dominantly located in the nucleus. In addition, this splicing variant is not only expressed in undifferentiated human embryonic stem cells but also expressed in the neural stem cell, with a similar expression pattern to that of SOX2.