Cytotoxic and apoptotic effect of chalcone 5 on mouse colon cancer cells CT-26

Abstract

According to World Health Organization data, various forms of cancer cause around 10 million deaths each year. Nearly 2 million new colon and rectal cancer cases were detected in 2020, making it the second greatest cause of cancer death. Aside from the harmful effects of various chemotherapeutic agents, a special difficulty for treating and eradicating these diseases is tumor resistance to existing anticancer medications and undesired side effects of chemotherapy against healthy cells and tissue. Chalcones are precursors for the synthesis of flavonoids and other compounds with a heterocyclic structure. The biological activities of this category of chemicals include antibacterial, anti-inflammatory, anticancer, antidiabetic, and antiproliferative properties. In the current study, we therefore examined the antitumor and apoptotic effects of chalcone 5 on mouse colon carcinoma cell line CT-26. Chalcone 5 caused the inner mitochondrial apoptotic pathway to be triggered in CT-26 cells by inducing apoptosis, disrupting the potential of the mitochondrial membrane and causing the release of cytochrome c into the cytosol. Based on results obtained in this study, chalcone 5 may be recommended as a potential promising anti-cancer candidate for future in vivo research studies utilizing experimental animals.

Based on results obtained in this study, chalcone 5 may be recommended as a potential promising anti-cancer candidate for future in vivo research studies utilizing experimental animals.