Network pharmacology and in vitro validation of anti-adipogenic property of Ipomoea mauritiana Jacq.

Abstract

Muscle wasting during starvation or malnourishment is a major health concern. Ipomoea mauritiana Jacq. is a plant from the Convolvulaceae family commonly known as Ksheera-vidari in Ayurveda which is prescribed in malnourishment and is known for imparting strength. It is also used as a galactogogue in lactating mothers. The study explores the possible mechanism of action of this herb. In silico studies using network pharmacology revealed AMPK, PPAR, longevity-regulating, insulin related and adipocytokine pathways were affected by the phytochemicals of this plant. Methanolic extract of Ipomoea mauritiana tuber was used for in vitro studies. The extract reduced lipid content in 3T3-L1 derived adipocytes. Gene expression studies revealed down-regulation of some key adipogenic genes such as PPARγ, Plin2, CD36 and FASN. Interestingly, treatment reduces RBP4 transcript level while an increase was observed in Adiponectin expression. This indicates that the extract reduces lipid content in adipocytes without increasing insulin resistance. This is the first report showing possible mechanism of action of Ipomoea mauritiana.