The comprehensive computational study on PE11 gene of Mycobacterium tuberculosis involved in virulence

Abstract

Since its initial discovery more than a decade ago, Mycobacterium tuberculosis has been the subject of significant scientific intrigue. The PE11 gene plays a pivotal role in mediating host-pathogen interactions within Mtb. Despite the fact that a comprehensive understanding of PE11 activities has yet to be achieved, evidence suggests that PE11 proteins play a role at various levels of the infectious process. According to several publications, PE11 (lipX) proteins, unique to pathogenic mycobacteria, are overexpressed during macrophage infection and inactive in TB patients. In this work, bioinformatics analysis was employed to anticipate the involvement of PE11 in mycobacterial virulence. The physicochemical characteristics, conserved domains, and theme of the obtained sequences were utilized to describe them structurally and functionally. Furthermore, homology modeling assisted in determining the 3-D structure and underlying residues in the active site area that interact with mshB and PPE41. Additionally, we reported on further research that may contribute to a more thorough understanding of the PE11 protein and its role in host-pathogen interactions. The findings presented here can be expanded upon and empirically confirmed.