Molecular Characterization of Phycobiliproteins Producing Cyanobacteria by using Nanopore Sequencer Isolated from Dairy Effluent

Abstract

Cyanobacteria can be used in so many different fields, such as wastewater treatment, biofuel production, nutraceuticals production, antioxidant production, human nutrition (spirulina) bio-fertilizer, and other areas. Cyanobacterial taxonomy has been established based on morphological features, such as the shape and dimensions of the cells, and the presence of structurally differentiated cells. Since, the last decade's molecular techniques were used for the identification of cyanobacteria based on 16S rRNA genes and it showed that they are a diverse, monophyletic phylum of organisms within bacterial radiation. Nanopore sequencing technology was the most recent technique for the identification of cyanobacterial species isolated from dairy effluent and screened based on effluent nutrients removal efficiency and phycobiliproteins production. The steps were followed by DNA extraction, library preparation, barcoding, DNA amplification, 16S rRNA sequencing by using MinION Nanopore sequencer and data analysis was evaluated by Epi2Me software for cyanobacterial species identification and preparation of the phylogeny. The two cyanobacterial species (Oscillatoria species-1 & Oscillatoria species-2) were molecularly identified as Oscillatoria acuminata and Oscillatoria sancta by 16S rRNA sequencing using Nanopore sequencing Technology and the DNA sequence was submitted to NCBI with stain name VMRJHK2020 and VMRJHK012020 and the Gene bank accession are MW364270 and MW364271, respectively.