Production, Optimization, Partial Purification, and Characterization of a Novel Cellulase from Bacillus subtilis 171ES and Its Potential for Use in Textiles

A NOVEL CELLULASE FROM BACILLUS SUBTILIS

Abstract

One hundred Bacillus sp. strains isolated from different soils were screened for cellulase production. A strain that produced maximum cellulase amounts was selected and identified by 16S rRNA analysis and was named Bacillus subtilis 171ES. In order to quantitatively determine the enzyme production capacity of the new isolate, it was grown in 3 different media. Optimization studies were carried out in the best medium. The results of nutritional optimization in the best liquid media showed that the best nitrogen and carbon sources, and metal ions were found to be milk powder, maltose and LiSO₄, respectively. In the study, enzyme production increased 6.6 times in the newly created modified medium. The enzyme was partially purified and characterized. The Optimum pH and temperature were 7 and 50°C, respectively. The BaCl₂ and MnSO₄ and ions were effective on activity. The kinetic values of the enzyme were V_max 63.7 U/mL and K_m 0.0064 mM. The molecular weight was estimated as 63 kDa. The potential of the enzyme for biostoning on denim fabric and removal of the cellulosic or vegetative impurities on wool fabric was evaluated. It was also found to be effective in fabrics. The cellulase enzyme of the new 171ES strain has the potential to be used in various textile processes.