The Determination of Pb(II) Ion with a Novel, Highly Efficient Immobilized Arsenazo III in a Polymer Matrix

Kholboeva  M. B; Smanova Z. A; Madatov U. A; Yusupov  Boburjon; A. K Nomozov; Sobirova  F. A.; Oybek Olimovich  Daminov; Mengboyev Otabek Alijonugli

doi:10.56042/jsir.v85i3.18988

Authors

Kholboeva M. B Department of Chemical Engineering, Termez State University of Engineering and Agrotechnologies https://orcid.org/0000-0003-0669-8676
Smanova Z. A Faculty of Chemistry, National University of Uzbekistan, Tashkent, 100 174, Uzbekistan https://orcid.org/0000-0003-3116-3518
Madatov U. A Faculty of Chemistry, National University of Uzbekistan, Tashkent, 100 174, Uzbekistan
Yusupov Boburjon Gulistan State University, Gulistan, Uzbekistan https://orcid.org/0009-0001-3356-5783
A. K Nomozov Department of Chemical Engineering, Termez State University of Engineering and Agrotechnologies. Termez, Uzbekistan https://orcid.org/0000-0003-0409-8247
Sobirova F. A. Faculty of Medicine, Department of Pharmacy and Chemistry, Alfrganus University, Tashkent, Uzbekistan https://orcid.org/0009-0005-6605-6247
Oybek Olimovich Daminov Internal Combustion Engines, Refrigeration and Cryogenic Technology, Tashkent State Technical University https://orcid.org/0000-0001-7876-1289
Mengboyev Otabek Alijonugli Faculty of pedagogy and social-humanitarian sciences, Foreign languages, Termez university of economics and service, Termez, 1901 11, Uzbekistan

DOI:

https://doi.org/10.56042/jsir.v85i3.18988

Keywords:

Direct ion determination, Sorption spectrophotometry, Lead detection, Polymer matrix, Water sample

Abstract

In this study, a novel analytical reagent based on arsenazo III immobilized on a polymer matrix composed of polyacrylonitrile, polyethylene polyamine, and dichloroethane (PPD) was developed and applied for the spectrophotometric determination of Pb(II) ions. The proposed method demonstrated standard analytical and metrological characteristics for the highly accurate determination of Pb(II) ions, as evidenced by a relative standard deviation (Sr) of 0.06 and a minimum detectable concentration (C_min) of 0.24 μg/L. The accuracy and reproducibility of the method were evaluated using Student’s t-test and Fisher’s F-test. Comparison of the experimental and tabulated values (experiment = 1.09; t_table = 2.83, F_experiment = 2.52; F_table = 4.47) confirmed the reliability of the proposed analytical procedure. At pH 5.8 in an acetate buffer medium, the Pb(II)–arsenazo III complex immobilized on the PPD matrix exhibited a maximum analytical signal at a wavelength of 640 nm. The measurement results followed the Beer–Lambert–Bouguer law, showing a linear relationship in the Pb(II) concentration range of 0.04-2.4 μg/mL. The method is simple, rapid, cost-effective, and allows direct ion determination in water samples with high sensitivity and selectivity.