Amphiphilic dendrimer loaded prussian blue nanoparticle for the detection of hydrogen peroxide

Abstract

A novel enzyme-free electrochemical sensor has been developed based on the prussian blue nanoparticles (PBNPs) loaded on a glassy carbon electrode (GCE) modified with amphiphilic poly(propylene imine) dendrimer (PBNPs/APPI(G3)/GCE) for sensing of hydrogen peroxide. The structural characterization of the newly synthesized template of APPI(G3) has been carried out by fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR) and matrix-assisted laser desorption/ionization coupled to time-of-flight mass spectroscopic (MALDI-TOF) studies. The electrodeposition and stabilization of PBNPs on the APPI(G3) template have been characterized by cyclic voltammetry and field emission scanning electron microscope (FESEM) studies. From the results, a homogeneous distribution of PBNPs with an average size of 50-100 nm on the APPI(G3) modified electrode surface has been observed. The PBNPs/APPI(G3)/GCE has shown an excellent performance towards the detection of hydrogen peroxide with ample electrochemical, mechanical stability, and good sensitivity to the other prussian blue-based H₂O₂ sensors. The developed sensor exhibit a linear response for H₂O₂ reduction over the concentration range of 100 to 1000 µM with a detection limit of 60 µM (S/N = 3), and sensitivity of 0.012 AM^-1 using the amperometric method. The obtained results have shown that PBNPs/APPI(G3)/GCE can be a promising electrochemical sensing platform for the detection of H₂O₂ in chemical and biological analysis.