Pretreatment strategy for enhanced lipid extraction from algal biomass in bio-oil production

Abstract

Efficient lipid extraction from algal biomass is a critical step in bio-oil production. Optimizing extraction parameters has led to the development of various methodologies; however, the primary challenge remains in effectively disrupting algal cells to facilitate lipid release. This study evaluates the impact of different pretreatment techniques on traditional lipid extraction methods, focusing on lipid extraction efficiency. Pretreatment methods examined include physical disruption (blending), irradiation (microwaving), and a novel combination of both. Traditional lipid extraction methods, such as Bligh-Dyer, Folch, and Soxhlet are employed. Results demonstrated that lipid extraction efficiency increased from 2.22–22.12% of dry weight (untreated biomass) to 10.09–52.08% of dry weight (pretreated biomass), varying across different algal species. The combined pretreatment (blender and microwave) followed by the Bligh-Dyer method yielded the highest lipid recovery. Chlamydomonas reinhardtii showed the maximum lipid content (52.08±2.22%). These findings highlight the significant enhancement in lipid recovery using the novel pretreatment and suggest its potential for improving bio-oil production.