Antibacterial, Antifungal, Antioxidant, and Molecular Docking Study of(E)-4-(1-(2-aminophenylimino)ethyl)benzene-1,3-diol

Abstract

The Schiff base,(E)-4-(1-(2-aminophenylimino)ethyl)benzene-1,3-diol (APEB) was synthesized and characterized by different spectroscopic techniques. The Schiff base, APEB show antibacterial and antifungal activities against a bacterium (E. coli) and a fungus (C.albicans) with an IC₅₀ value of Approx 0.07µg/ml and Approx 0.06µg/ml respectively.  Clear zones were not observed in the case of antibacterial activity but present in the case of antifungal activity. Additionally, antioxidant potential was evaluated through DPPH, ABTS, FRAP, CUPRAC, SOARSA, RNOSA and HFRSA, with an IC₅₀ values of 187.2 µg/ml, 5.967 ± 0.1625 µg/ml, 0.7404 µg/ml, 2.37 µg/ml, above maximum dose limit i.e. higher than 1000µg/ml, 167.6, 9.934 indicating antioxidant properties. To elucidate the potential mechanisms underlying these bioactivities, molecular docking studies were performed against the protein: PDB ID 1AI6 and 5AEZ.